Set reference scale (step 1)
Set the scale of the picture in order for the software to provide measurements in the adequate unit of measure.
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In the ribbon, click on the Measure distance button to establish a matching between pixels number and real size (in micro-meters for example).
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Measure a reference scale bar using the mouse.Click on first pixel of the scale bar, hold the left-button of the mouse, drag it to the end of the scale bar and release the left-button of the mouse.
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A new line is now added to the Pixels measurements grid where the number of measured pixels is recorded.Enter the real size in the Distance column and add a name to the scale.
This information will be saved and can therefore be used again in the future for pictures taken using the same scaling factor. -
To remove a scale from the pixels measurement grid, highlight it and hit the Delete key of the keyboard.
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When the correct scale is recorded in the Pixels measurements grid, highlight it and click on the Next button in the ribbon.