Align two sequences
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To align two sequences, under Molecular Tools, in the Pairwise alignment group, click Align two sequences.
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Paste the first sequences in the textbox for First sequence.
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Use the Clear sequence button
to clear the sequence from unwanted characters. -
Use the Reverse-complement button
to reverse and complement the sequence. -
Paste the second sequence in the textbox for Second sequence.
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Click on the small arrow button on the right of the Align button to activate the right algorithm and sequence type for this alignment.
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Algorithm options
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BioloMICS Alignment Algorithm
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BioloMICS - Smith Waterman Full expansion with Smith Waterman
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BioloMICS strict No expansion between identical blocks
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Blast
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Sequence type options: DNA sequence or Protein sequence.
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Click the Align button to start the alignment and to see the results in the textbox at the bottom of the window.
This movie shows how perform a pairwise sequence alignment in BioloMICS.
1. Molecular tools - Align two sequences (0:10)
2. Paste sequence 1 and 2 (0:17)
3. Revert and clean (0:27)
4. Select algorithm and type (0:34)
5. Align (0:41)